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KMID : 0364820080440020122
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Korean Journal of Microbiology
2008 Volume.44 No. 2 p.122 ~ p.129
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Antibacterial Synergic Effect and Cellular Responses of Nalidixic Acid-Resistant Salmonella typhimurium Exposed to Tea Polyphenols and Nalidixic Acid
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Lim Ye-Ji
Cho Yun-Seok
Oh Kye-Heon
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Abstract
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The purpose of this work was to investigate the synergically bactericidal effects and cellular responses of green
tea polyphenols (TPPs) and nalidixic acid (NA) on nalidixic acid-resistant (NAR) Salmonella typhimurium. The
bactericidal activities of >3,500 μg/ml TPPs and <256 μg/ml NA were investigated for S. typhimurium of
which initial cell number was approximately adjusted to 107 cell/ml. Complete elimination of NAS-S. typhimurium
was achieved within 6 hr of incubation at the concentrations of 3,500 μg/ml TPP or 256 μg/ml NA,
whereas only partial bactericidal effect was achieved under the same conditions. However, the combinations of
3,000 μg/ml TPPs and 32 μg/ml NA against NAS-S. typhimurium and 3,500 μg/ml TPPs and 64 μg/ml nalidixic
acid against NAR-S. typhimurium showed complete removal within 5 hr of incubation. The stress shock
proteins (SSPs) were induced at different concentrations of TPP or NA used as stressors against cell culture of S.
typhimurium. The proteins were identified as 70-kDa DnaK and 60-kDa GroEL by SDS-PAGE and Western
blot. SSPs induced by the stressors were found to increase in proportion to the TPPs or NA. Scanning electron
microscopy analyses revealed the presence of perforations and irregular rod shape with wrinkled surfaces for
cells treated with TPPs or NA.
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KEYWORD
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nalidixic acid, S. typhimurium, synergic effect, tea polyphenols
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